In vitro production of zygote from slaughterhouse driven buffalo oocyte

Effect of Leptin on In Vitro Nuclear Maturation and Apoptosis of Buffalo (Bubalus bubalis) Oocyte

IN VITRO PRODUCTION OF BUFFALO (Bubalus bubalis) EMBRYOS

Two experiments (Exp) were carried out to test the effect of different media on in vitro maturation (IVM) (Exp 1; n = 1142 ovaries with 2666 oocytes) and to study in vitro fertilization (IVF) of buffalo oocytes (Exp 2; n = 110 ovaries with 330 oocytes). In exp 1 the collected cumulus oocyte complex (COCs) was allocated to three maturational media; TCM-199 supplemented with hormones (M1), TCM-19...

Embryological Aspects of Oocyte In Vitro Maturation

In Vitro Maturation (IVM) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. This review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. The review is based on literature reports and the author’s experience. In IVM cycles,...

Effect of Leptin on In Vitro Nuclear Maturation and Apoptosis of Buffalo (Bubalus bubalis) Oocyte

BACKGROUND Leptin, as a 16 kDa adipokine, is a pleiotropic cytokine-like hormone that primarily secreted from adipose tissue. It also involves in the regulation of energy homeostasis, neuroendocrine function, immunity, lipid and glucose homeostasis, fatty acid oxidation, angiogenesis, puberty and reproduction. The aim of this study was to investigate the effects of in vitro addition of leptin t...

Survival of Vitrified Water Buffalo Cumulus Oocyte Complexes and Their Subsequent Development in Vitro

The study aimed to determine the effects of different concentrations of glycerol and ethylene glycol and ultrarapid cryopreservation (vitrification) on survival and subsequent in vitro development of bubaline cumulus oocyte complexes (COCs) in order to recognize the optimum cryoprotectant. Survival and in vitro maturation, fertilization and cleavage of buffalo COCs was evaluated subsequent to t...